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rhodamine labelled wga lectin  (Vector Laboratories)


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    Vector Laboratories rhodamine labelled wga lectin
    Treatment with T3 counteracts pathological growth in diabetes- and glucose-injured podocytes and cardiomyocytes and maintains cardiomyocytes cytoarchitecture (A and B) Representative images of Glepp1 (A) and WT1 (B) staining in kidney serial sections of lean and diabetic animals. Quantification of individual podocyte volume and WT1-positive podocytes in lean and ZDF rats (A and B, right). (C) Quantification of Feret’s diameter in cardiomyocytes. (D and E) Evaluation of DNA content in glomerular cells (D) and cardiomyocytes (E) of control and diabetic animals. (F) Representative images of 3D reconstructed kidney (top) and heart (bottom) tissue sections. Tissues were stained with rhodamine-labeled <t>WGA</t> <t>lectin.</t> (G–I) Quantification of cell area (G, H) and axis ratio (I) in control, glucose-injured, and T3-treated human podocytes (G) and cardiomyocytes (H, I). For quantification, representative images were randomly taken from different areas of cell culture. At least 91 cells from each group were analyzed from n = 3 independent experiments (G–I). Data are expressed as mean ± SEM, one-way ANOVA corrected with Tukey’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. n = 5–6 animals per group. Scale bars, 20 μm (A, B), 40 μm (F).
    Rhodamine Labelled Wga Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhodamine+labelled+wga+lectin/Rhodamine+labeled+Wheat+Germ+Agglutinin+(WGA)/pmc10518716-37-0-4
    Average 96 stars, based on 266 article reviews
    rhodamine labelled wga lectin - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs"

    Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs

    Journal: iScience

    doi: 10.1016/j.isci.2023.107826

    Treatment with T3 counteracts pathological growth in diabetes- and glucose-injured podocytes and cardiomyocytes and maintains cardiomyocytes cytoarchitecture (A and B) Representative images of Glepp1 (A) and WT1 (B) staining in kidney serial sections of lean and diabetic animals. Quantification of individual podocyte volume and WT1-positive podocytes in lean and ZDF rats (A and B, right). (C) Quantification of Feret’s diameter in cardiomyocytes. (D and E) Evaluation of DNA content in glomerular cells (D) and cardiomyocytes (E) of control and diabetic animals. (F) Representative images of 3D reconstructed kidney (top) and heart (bottom) tissue sections. Tissues were stained with rhodamine-labeled WGA lectin. (G–I) Quantification of cell area (G, H) and axis ratio (I) in control, glucose-injured, and T3-treated human podocytes (G) and cardiomyocytes (H, I). For quantification, representative images were randomly taken from different areas of cell culture. At least 91 cells from each group were analyzed from n = 3 independent experiments (G–I). Data are expressed as mean ± SEM, one-way ANOVA corrected with Tukey’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. n = 5–6 animals per group. Scale bars, 20 μm (A, B), 40 μm (F).
    Figure Legend Snippet: Treatment with T3 counteracts pathological growth in diabetes- and glucose-injured podocytes and cardiomyocytes and maintains cardiomyocytes cytoarchitecture (A and B) Representative images of Glepp1 (A) and WT1 (B) staining in kidney serial sections of lean and diabetic animals. Quantification of individual podocyte volume and WT1-positive podocytes in lean and ZDF rats (A and B, right). (C) Quantification of Feret’s diameter in cardiomyocytes. (D and E) Evaluation of DNA content in glomerular cells (D) and cardiomyocytes (E) of control and diabetic animals. (F) Representative images of 3D reconstructed kidney (top) and heart (bottom) tissue sections. Tissues were stained with rhodamine-labeled WGA lectin. (G–I) Quantification of cell area (G, H) and axis ratio (I) in control, glucose-injured, and T3-treated human podocytes (G) and cardiomyocytes (H, I). For quantification, representative images were randomly taken from different areas of cell culture. At least 91 cells from each group were analyzed from n = 3 independent experiments (G–I). Data are expressed as mean ± SEM, one-way ANOVA corrected with Tukey’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. n = 5–6 animals per group. Scale bars, 20 μm (A, B), 40 μm (F).

    Techniques Used: Staining, Control, Labeling, Cell Culture


    Figure Legend Snippet:

    Techniques Used: Recombinant, Plasmid Preparation, Membrane, Enzyme-linked Immunosorbent Assay, DC Protein Assay, Software

    Related Articles

    Whole Genome Amplification:

    Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs
    Article Snippet: FITC wheat-germ agglutinin (WGA) , Vector Laboratories , Cat#FL1021. .. Rhodamine-labelled WGA lectin , Vector Laboratories , Cat#RL1022. .. FITC-conjugated-phalloidin , Life Technologies , Cat#A12389.

    Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs
    Article Snippet: After fixing thick OCT-frozen tissue sections in 4% PFA for 10 minutes, they were permeabilized with methanol (Cat#34860, Sigma) for 30 minutes at RT. .. Kidney and left ventricle sections were then incubated overnight at 4°C with rhodamine-labelled WGA lectin (Cat#RL1022, Vector Laboratories, 1:400) in DAPI (Cat#D9542, Sigma-Aldrich, 1 μg/ml) to visualize cell membranes and stain nuclei, respectively. .. To visualize DNA content in beta cells, pancreatic sections were incubated overnight at 4°C with rabbit anti-insulin antibody (Cat#sc-9168, Santa Cruz Biotechnology, 1:100) in DAPI (Cat#D9542, Sigma-Aldrich, 1 μg/ml) and, after washing with PBS, incubated for 4 hours at RT with FITC-conjugated donkey anti-rabbit secondary antibody (Cat#711-095-152, Jackson Immuno Research, 1:100).

    Incubation:

    Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs
    Article Snippet: After fixing thick OCT-frozen tissue sections in 4% PFA for 10 minutes, they were permeabilized with methanol (Cat#34860, Sigma) for 30 minutes at RT. .. Kidney and left ventricle sections were then incubated overnight at 4°C with rhodamine-labelled WGA lectin (Cat#RL1022, Vector Laboratories, 1:400) in DAPI (Cat#D9542, Sigma-Aldrich, 1 μg/ml) to visualize cell membranes and stain nuclei, respectively. .. To visualize DNA content in beta cells, pancreatic sections were incubated overnight at 4°C with rabbit anti-insulin antibody (Cat#sc-9168, Santa Cruz Biotechnology, 1:100) in DAPI (Cat#D9542, Sigma-Aldrich, 1 μg/ml) and, after washing with PBS, incubated for 4 hours at RT with FITC-conjugated donkey anti-rabbit secondary antibody (Cat#711-095-152, Jackson Immuno Research, 1:100).

    Staining:

    Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs
    Article Snippet: After fixing thick OCT-frozen tissue sections in 4% PFA for 10 minutes, they were permeabilized with methanol (Cat#34860, Sigma) for 30 minutes at RT. .. Kidney and left ventricle sections were then incubated overnight at 4°C with rhodamine-labelled WGA lectin (Cat#RL1022, Vector Laboratories, 1:400) in DAPI (Cat#D9542, Sigma-Aldrich, 1 μg/ml) to visualize cell membranes and stain nuclei, respectively. .. To visualize DNA content in beta cells, pancreatic sections were incubated overnight at 4°C with rabbit anti-insulin antibody (Cat#sc-9168, Santa Cruz Biotechnology, 1:100) in DAPI (Cat#D9542, Sigma-Aldrich, 1 μg/ml) and, after washing with PBS, incubated for 4 hours at RT with FITC-conjugated donkey anti-rabbit secondary antibody (Cat#711-095-152, Jackson Immuno Research, 1:100).



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    Treatment with T3 counteracts pathological growth in diabetes- and glucose-injured podocytes and cardiomyocytes and maintains cardiomyocytes cytoarchitecture (A and B) Representative images of Glepp1 (A) and WT1 (B) staining in kidney serial sections of lean and diabetic animals. Quantification of individual podocyte volume and WT1-positive podocytes in lean and ZDF rats (A and B, right). (C) Quantification of Feret’s diameter in cardiomyocytes. (D and E) Evaluation of DNA content in glomerular cells (D) and cardiomyocytes (E) of control and diabetic animals. (F) Representative images of 3D reconstructed kidney (top) and heart (bottom) tissue sections. Tissues were stained with rhodamine-labeled <t>WGA</t> <t>lectin.</t> (G–I) Quantification of cell area (G, H) and axis ratio (I) in control, glucose-injured, and T3-treated human podocytes (G) and cardiomyocytes (H, I). For quantification, representative images were randomly taken from different areas of cell culture. At least 91 cells from each group were analyzed from n = 3 independent experiments (G–I). Data are expressed as mean ± SEM, one-way ANOVA corrected with Tukey’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. n = 5–6 animals per group. Scale bars, 20 μm (A, B), 40 μm (F).
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    Image Search Results


    Treatment with T3 counteracts pathological growth in diabetes- and glucose-injured podocytes and cardiomyocytes and maintains cardiomyocytes cytoarchitecture (A and B) Representative images of Glepp1 (A) and WT1 (B) staining in kidney serial sections of lean and diabetic animals. Quantification of individual podocyte volume and WT1-positive podocytes in lean and ZDF rats (A and B, right). (C) Quantification of Feret’s diameter in cardiomyocytes. (D and E) Evaluation of DNA content in glomerular cells (D) and cardiomyocytes (E) of control and diabetic animals. (F) Representative images of 3D reconstructed kidney (top) and heart (bottom) tissue sections. Tissues were stained with rhodamine-labeled WGA lectin. (G–I) Quantification of cell area (G, H) and axis ratio (I) in control, glucose-injured, and T3-treated human podocytes (G) and cardiomyocytes (H, I). For quantification, representative images were randomly taken from different areas of cell culture. At least 91 cells from each group were analyzed from n = 3 independent experiments (G–I). Data are expressed as mean ± SEM, one-way ANOVA corrected with Tukey’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. n = 5–6 animals per group. Scale bars, 20 μm (A, B), 40 μm (F).

    Journal: iScience

    Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs

    doi: 10.1016/j.isci.2023.107826

    Figure Lengend Snippet: Treatment with T3 counteracts pathological growth in diabetes- and glucose-injured podocytes and cardiomyocytes and maintains cardiomyocytes cytoarchitecture (A and B) Representative images of Glepp1 (A) and WT1 (B) staining in kidney serial sections of lean and diabetic animals. Quantification of individual podocyte volume and WT1-positive podocytes in lean and ZDF rats (A and B, right). (C) Quantification of Feret’s diameter in cardiomyocytes. (D and E) Evaluation of DNA content in glomerular cells (D) and cardiomyocytes (E) of control and diabetic animals. (F) Representative images of 3D reconstructed kidney (top) and heart (bottom) tissue sections. Tissues were stained with rhodamine-labeled WGA lectin. (G–I) Quantification of cell area (G, H) and axis ratio (I) in control, glucose-injured, and T3-treated human podocytes (G) and cardiomyocytes (H, I). For quantification, representative images were randomly taken from different areas of cell culture. At least 91 cells from each group were analyzed from n = 3 independent experiments (G–I). Data are expressed as mean ± SEM, one-way ANOVA corrected with Tukey’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. n = 5–6 animals per group. Scale bars, 20 μm (A, B), 40 μm (F).

    Article Snippet: Rhodamine-labelled WGA lectin , Vector Laboratories , Cat#RL1022.

    Techniques: Staining, Control, Labeling, Cell Culture

    Journal: iScience

    Article Title: Thyroid hormone treatment counteracts cellular phenotypical remodeling in diabetic organs

    doi: 10.1016/j.isci.2023.107826

    Figure Lengend Snippet:

    Article Snippet: Rhodamine-labelled WGA lectin , Vector Laboratories , Cat#RL1022.

    Techniques: Recombinant, Plasmid Preparation, Membrane, Enzyme-linked Immunosorbent Assay, DC Protein Assay, Software